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首页> 外文OA文献 >High Resolution Genetic Mapping by Genome Sequencing Reveals Genome Duplication and Tetraploid Genetic Structure of the Diploid Miscanthus sinensis
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High Resolution Genetic Mapping by Genome Sequencing Reveals Genome Duplication and Tetraploid Genetic Structure of the Diploid Miscanthus sinensis

机译：基因组测序的高分辨率遗传图谱揭示了二倍体中华（Miscanthus sinensis）的基因组重复和四倍体遗传结构。

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We have created a high-resolution linkage map of Miscanthus sinensis, using genotyping-by-sequencing (GBS), identifying all 19 linkage groups for the first time. The result is technically significant since Miscanthus has a very large and highly heterozygous genome, but has no or limited genomics information to date. The composite linkage map containing markers from both parental linkage maps is composed of 3,745 SNP markers spanning 2,396 cM on 19 linkage groups with a 0.64 cM average resolution. Comparative genomics analyses of the M. sinensis composite linkage map to the genomes of sorghum, maize, rice, and Brachypodium distachyon indicate that sorghum has the closest syntenic relationship to Miscanthus compared to other species. The comparative results revealed that each pair of the 19 M. sinensis linkages aligned to one sorghum chromosome, except for LG8, which mapped to two sorghum chromosomes (4 and 7), presumably due to a chromosome fusion event after genome duplication. The data also revealed several other chromosome rearrangements relative to sorghum, including two telomere-centromere inversions of the sorghum syntenic chromosome 7 in LG8 of M. sinensis and two paracentric inversions of sorghum syntenic chromosome 4 in LG7 and LG8 of M. sinensis. The results clearly demonstrate, for the first time, that the diploid M. sinensis is tetraploid origin consisting of two sub-genomes. This complete and high resolution composite linkage map will not only serve as a useful resource for novel QTL discoveries, but also enable informed deployment of the wealth of existing genomics resources of other species to the improvement of Miscanthus as a high biomass energy crop. In addition, it has utility as a reference for genome sequence assembly for the forthcoming whole genome sequencing of the Miscanthus genus.
机译：我们使用基因分型（GBS）方法创建了中华芒（Miscanthus sinensis）的高分辨率连锁图谱，首次确定了全部19个连锁群。由于芒草具有非常大且高度杂合的基因组，但迄今为止尚无或仅有有限的基因组信息，因此该结果在技术上具有重要意义。包含两个父母亲连锁图中的标记的复合连锁图由3745个SNP标记组成，在19个连锁组上跨越2,396 cM，平均分辨率为0.64 cM。对中华高粱（M.sinensis）复合连锁图与高粱，玉米，水稻和短枝曲霉的基因组进行的比较基因组学分析表明，与其他物种相比，高粱与芒草的同系关系最紧密。比较结果显示，除了LG8定位到两个高粱染色体（4和7）外，LG M的每对19个中华。链均与一个高粱染色体对齐，这大概是由于基因组复制后发生的染色体融合事件。数据还揭示了相对于高粱的其他几种染色体重排，包括中华绒螯蟹LG8中高粱同系染色体7的两个端粒-着丝粒倒置和中华绒螯蟹LG7和LG8中高粱同系染色体4的两个副中心倒置。结果首次清楚地表明，二倍体中华按蚊是四倍体起源，由两个亚基因组组成。这种完整而高分辨率的复合连锁图谱不仅可以用作新的QTL发现的有用资源，而且还可以在知情的情况下部署其他物种现有的丰富基因组资源，以改善作为高生物量能源作物的芒草。另外，它对于即将进行的芒草属全基因组测序的基因组序列组装具有参考价值。

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